1. What is the difference between a genomic and a cDNA library? What library would you want to screen if you needed to find a protein-encoding gene?

What is the difference between a genomic and a cDNA library? What library would you want to screen if you needed to find a protein-encoding gene?

2. What is the purpose of Sanger sequencing? What is the reason the ddNTPs used lack a 3’-hydroxyl group?

4. The questions below are based on RT-PCR:

a. How is mRNA purified from cells?

b. How does reverse transcriptase make cDNA? How is cDNA different that DNA?

 
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