Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?

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Exercise4: Purebacterialcolonies

1. Whenanagarplateis inoculated,whyis theloopsterilizedaftertheinitialinoculumis puton?

2. Distinguishbetweena purecultureandamixedculture.

3. Definea bacterialcolony.Listfourcharacteristicsbywhichbacterialcoloniesmaybe distinguished.

4. Whyshoulda Petridishnotbe leftopenforanyextendedperiod?

5. Whydoesthestreakingmethod youusedto inoculateyourplatesresultin isolatedcolonies?

Exercise5: Pourplateandstreakingtechniquetoobtainpurecultures

1. Discusstherelativeconvenienceofpour-andstreak-platetechniquesinculturingclinical specimens.

2. Howdoyoudecidewhichcoloniesshouldbepickedfrom aplatecultureofamixedflora?

3. Whyisitnecessarytomakepuresubculturesoforganismsgrownfrom clinicalspecimens?

4. Whatkindsofclinicalspecimensmayyielda mixedflorainbacterialcultures?

5. Whenmorethanonecolonytypeappearsinpureculture,whatarethemostlikelysourcesof extraneouscontamination?

Exercise3: Primarymediaforisolationofmicroorganisms

1.Definea differentialmediumanddiscussitspurpose.

2.Definea selectivemediumanddescribeitsuses.

3.WhyisMacConkeyagarselectiveas wellasdifferential?

4.Whyisbloodagarusefulasaprimaryisolationmedium?

5.Whatis themajordifferencebetweenModifiedThayer-Martin(MTM)andchocolateagar?Whenwould youuseMTMratherthanchocolateagar?

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